Borate buffer calculator
Borate buffer calculator. 3014 M. 6) CHES (0. This helps to slow the rise in pH, making it easier to contain pH. 20 M in total borate. BBS has many uses because it is isotonic and has a strong bactericidal effect. 127-0. For over 25 years we have been a US and Worldwide Distributor / Supplier of over 300,000 lab supplies, glassware, laboratory equipment and related consumables. Pack: 500 mL. Adjust the pH to 8. This buffer contains 0. Dissolve the ingredients in H 2 O. According to this source , zwitterions have no net contribution to ionic strength, so that HEPES produces only a very small (though likely still non-zero) effect on ionic strength. 5 mL cells + 0. TBE is one of the very common electrophoresis buffers, used for agarose gel analysis of DNA. Add 8 g of Sodium chloride to the solution. The first step is to test the current borate level in your swimming pool. 40 mol NH 4 Cl are mixed to give 1 L of solution. Most protonated component: 0 g/L, 0 mM. Perhaps the most elegant method is to use sodium tetraborate also known as borax (Na 2 B 4 O 7 ·10H 2 O) and H 3 BO 3. Measure dry weight of sample on a separate portion. Tris buffer is one of the most commonly used buffer systems in biology labs. 187 M NH4Cl when 20. Sodium Hydroxide, 6N Dissolve 24 g NaOH in distilled water and dilute up to 100 mL. What follows are some of the most impactful reasons to treat your water with borate. They function as a pH buffer and an algaecide, helping to maintain water balance and prevent algae growth. Buffer Calculator is an online tool for buffer pH calculations. Thus, after hydrolysis, the next step in an amino acid analysis is derivatization. Applications. 0672 Microparticle buffers should be suitable for both the ligand and the task at hand. For example if a system contains both CH3COOH and CH3COONa then the pH of this buffer can be calculated. ddH 2 O. 1 N NaOH solution to 500 mL approximately 0. Warning: For laboratory use only. 2 - [bori …. 2 m boric acid) 50 mL: Solution B (0. This was dependent on borate ion concentration at constant pH. This buffer calculator provides empirical formula, pKa, buffer pH range, and formula weight. We would like to show you a description here but the site won’t allow us. Notes: This product was formerly listed as BB-159. Store at room temperature. 170 M NH3 and 0. , Acros Organics 1303-96-4) in 100 mL distilled H 2 O. 0) Procedure. This section describes the preparation of protein or peptide hydrolysates for derivatization using Waters AccQ•Tag chemistry. 05 g of Sodium bicarbonate to the solution. 52 is followed by accurate a … buffer pH Calculator: Input the pK a values, the desired pH, and buffer strength Get recipes and other important info about your buffer Enter pK a values, . g. 52) provided a molar extinction coefficient of ε = 12544. 0) Citrate Buffer (pH 3. 29 (Ubuntu) Server at labchem. A simple buffer system might be a 0. 8 g of sodium tetraborate decahydrate (e. 0). I recommend keeping your borate level around 30-50 ppm (parts per million). 0 mL of 0. Water. Boric acid has a pK a of 9. 2, the more resistance borates will provide. 5 mg of Acetic Acid to the solution. 6 to 8. The software-. 2-7. 15 M, pH 5. Our borate buffer solutions, available with a pH of 8. 44 g of Sodium Phosphate Dibasic to the solution. 4). I have developed it just for you so that you can calculate the required amount of individual 9. Best Prices! Borate Buffer, 0. Sodium Borate Buffer is a buffer that is used to maintain the pH within a relatively narrow range. 05 M. pH: 8. 0) (or 9. Now let's apply this to swimming pools. Apr 24, 2020 · To calculate the specific pH of a given buffer, you need to use the Henderson-Hasselbalch equation for acidic buffers: “pH = pKa + log10([A-]/[HA]),” where Ka is the “dissociation constant” for the weak acid, [A-] is the concentration of conjugate base and [HA] is the concentration of the weak acid. Pool Calculator provides all the tools and information you need to keep your swimming pool and hot tub chemistry balanced and your water crystal clear. 5 mg. The thermal lability of 2-(2′ 4′-dinitrophenylamino)-2-deoxy-D-glucose (DNP-glucosamine) in alkaline borate buffer is reported. Note: The assay can be performed using other buffers, provided they are free of primary amines (e. Adjust the pH to 6. Concentrations can be calculated by molarity or percentage. Borate buffer suitable for HPLC, LiChropur™, 0. Previous Buffers 9025 Technology Dr. 22-μm filter. Jun 19, 2014 · Measure the OD 600 of the resuspended cells (blank against Z buffer) Dilute cells in Z buffer to 1 mL (most easily done with a pippeter). Filter sterilize and store at room temperature. Add 95 g of Potassium Phosphate Monobasic to the solution. Borate does a great job regulating your water and keeping it well balanced. 50. 20. 50 ± 0. Sodium borate salts (such as borax) can also be utilized as substitutes for boric acid and have similar properties and functions. 5, are ideal for maintaining a narrow pH range during various biochemical techniques. 1 liter/mole, which was a much lower numerical value than that for the other buffering systems, and the spectrum obtained did not resolve a We would like to show you a description here but the site won’t allow us. Tris-acetate-EDTA (TAE) running buffer and tris-borate-EDTA (TBE) are commonly used buffers for DNA agarose gel electrophoresis that are especially useful in preparative work. 4. 005871 M. 5 with 6 N NaOH, using a pH Dissolve 152. 10 , the value of α is obtained in accordance with Eq. Nov 7, 2019 · Use these simple instructions to make stock and working solutions of TBE (tris-borate-EDTA) buffer for gel electrophoresis separation of DNA. Borate buffers have an alkaline buffering capacity in the 8–10 range. rec8458 ). 0, 1×) Reagent Volume per 500 mL of solution (v/v) Solution A (0. 1 and CYA of 0, 30, 50 or 100 ppm and Borate of 0, 30 and 50 pm. Add 3 mL borate buffer and adjust to pH 9. 2 g of Potassium Chloride to the solution. The BSA blocking buffer recipe calculator enables the accurate preparation of BSA blocking solution whether you are making enough for a single experiment or for the entire lab. 721 g of Sodium Acetate to the solution. The boric acid-borate CA. Ammonia removal: Pipet 50 mL sample or an appropriate volume diluted to 50 mL with water into a 100-mL beaker. com 800. 3 g Na2EDTA) to the solution. (0. Add 9. To make 100 mL of a 1% BSA blocking buffer, dissolve 1 g of BSA in 100 mL of TBST. 3 ( cshlp-pdb. Sodium tetraborate (25 mM) 38. View the full answer. Calculate the pH of a buffer solution that contains 0. 2M solution = 12. The thicker films might be attributed to longer time of potentiostatic polarization. 70. Like CYA, borates contribute to Total Alkalinity (TA), and therefore must also be deducted when calculating the water's carbonate alkalinity (or corrected alkalinity), which is required for the LSI equation. 0 that is 0. It also P. Add 1. $\begingroup$ I think calculation of the ionic strength may be complicated due to the zwitterionic character of HEPES. 1. You can use simple borate test strips to find that out. 0 to 8. Approximate pH values are calculated with Henderson-Hasselbalch equation. Add deionized water to 1L. 15, 7. 4. 80 - $55. Solutions. To make a purchase inquiry for this buffer, please provide your email address below: Request quotation. 5 mL Z buffer will produce a desirable amount of yellow color in 1-2 hours. Use the Henderson - Hasselbalch equation to calculate the ratio of H3. Reagents. Adjust solution to desired pH using HCl (typically pH ≈ 7. Borates at 50 ppm provide as much protection from pH rise as 221 ppm of TA provides against pH Sep 2, 2014 · The borate buffer system (pH 7. 77 g. 68. Component Name. bangslabs. Borate Helps Prevent Algae This fact about borate is 100% true! Borate serves as a buffer for the pool. 5 g sodium tetra borate and dilute to 1000 mL with distilled water. 44 g. To use the calculator Apr 24, 2017 · Borate buffers function from pH 8. Fishers, IN 46038 • www. Volume per 500 mL of solution (v/v) Solution A (0. It also provides a list of pKa values of buffers commonly used in biology and biochemistry. CB. 2) Citrate-Phosphate Buffer (0. Prepare 800 mL of distilled water in a suitable container. Compared to tris-borate-EDTA (TBE) and tris-phosphate-EDTA (TPE) buffers, double-stranded DNA tends to run faster in TAE. Storage: Room Temp. $\endgroup$ Calculate the pH of a buffer that is 0. CAPS (0. Sign up to receive technical advice and special offers straight to your inbox. SKU: 11455-90. 95 g. Recipe can be automatically scaled by entering desired final volume. 0. $34. To make it pH 9. 00 mol NH 3 and 0. From the plot of V c vs log( a C l − ), as shown in Fig. 1 N Sodium hydroxide (4g NaOH/L) to 500 mL 0. Boston BioProducts offers a comprehensive range of biological buffers for use in research, diagnostics, drug development, and manufacturing. 24 Given that [boric acid] + [sodium borate] = 0. 5) preparation guide and recipe. Amino acid analysis by liquid chromatography and optical detection requires additional sample preparation, because most amino acids lack a chromophore and cannot be detected. 66380 Slide-A-Lyzer Dialysis Cassettes, 10,000MWCO . Adjust the volume to 1 liter using distilled water. 5 M H3BO3 and 0. In this article, you learn how to prepare TBE step-by-step. Mon - Fri: 8am - 8pm ET. the toxicity of the buffer. 2 to 10. com Port 80 Concentration. 5 M NaH2BO3? You want pH = 9. Buffer Calculation. This is the best phosphate buffer calculator available elsewhere online. 05 m sodium tetraborate 10H 2 O) 200 mL. 5x working solution. 125 M Sodium tetraborate mixed in fixed ratio to attain the desired pH strength. 1 m) Dissolve 3. Phosphate Buffer Citric Acid Buffer. Unlike other inorganic oxygen-containing acids, boric acid is a Lewis acid, and its acidity through the For your convenience, the biological buffers table contain values of pKa 0, d(pKa 0)/dt at 298. Amino acid buffers such as glycine and histidine perform over a diversity of pH ranges. Dissolve Tris in about 800 mL of deionized water. 8). 5 M Boric acid and 0. 52 conditions. 100% (1 rating) The pKa of boric acid = 9. Select TBE Borate Buffer. Total acidity is the same concept as total alkalinity but total acidity buffers pH rise whereas total alkalinity buffers pH drop. The physical and chemical properties of the boric acid and its borate salts are shown in the table below: APPLICATION Borate buffered saline (abbreviated BBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range. To make a purchase inquiry for this buffer, please provide Aug 20, 2021 · Figure 8. Add 2. 3 you must calculate that the buffer must contain H3BO3 and H2BO3-As a quick question : —-What would be the buffer pH if it was produced from 0. It is isotonic and has a strong bactericidal effect, and can be used to dilute substances or in coating procedures. The solution is sterilized by autoclaving and is stored at room temperature. Sodium Borate Buffer (0. 0 with buffer capacity of 0. As your pH naturally rises, the closer it gets to 9. In addition, buffer solutions, buffer tablets, and buffer solids may be obtained from commercial sources in convenient prepackaged form. 0) Citrate-Phosphate Buffer (110 mM, pH 5. the buffering capacity in the desired pH range with the ability to maintain constant pH during fixation. buffer pH Calculator: Input the pK a values, the desired pH, and buffer strength Get recipes and other important info about your buffer Enter pK a values, separated Jun 24, 2017 · The phosphate buffer calculator is based on this Henderson Hasselbalch equation and can be employed to calculate the concentration of individual components of a buffer system. (Refer to Martin’s page 171for Example 8-6, and page 175 for preparation, 7th Ed. Apr 3, 2023 · Borates are chemical compounds derived from boron, a naturally occurring element found in rocks, soil, and water. 605 g. 6) Citrate-Phosphate Buffer (40 mM, pH 5. Borate Buffer (pH 9. Here’s the best way to solve it. Optimal pH is 8. 24) and sodium borate. 703 g of Sodium Citrate dihydrate to the solution. 26 M Na2HPO4. EDTA does not have any essential function in electrophoresis buffers but it is added 9. 9 mL Z buffer. 5 gm Na 2 B 4 O 7. 22858 Fluorescent Dye Removal Columns. a. The discussion presented here is by no means complete, but we hope it will help in the understanding of general Borate is a buffer against a rise in pH, much like carbonate alkalinity is a buffer against a reduction in pH. View Product. The buffers are prepared fresh for each order and conveniently packaged. This booklet is designed to help answer basic questions about the use of buffers in biological systems. ). Tris-borate provides electrical conductivity as well as maintains the pH of the buffer. 14 5. Calculate the pH of a buffer solution that consists of 0. Least protonated component: 0 g/L, 0 mM. 325 M solution of pyridine hydrofloride? 0 How to calculate the composition of a borate buffer with a defined pH using the Henderson-Hasselbalch equation? Sodium borate is used as anhydrous borax for proteomics research, used in the manufacturing of glazes and enamels, a component in tanning agents; curing and preserving skins, a agent used in cleaning compounds, used to artificially age wood, as a preservative, either alone or with other antiseptics 352. 2 m boric acid) 50 mL. Add 121. 387. 7. Add dH2O until the volume is 1 L. To make a purchase inquiry for this buffer, please provide your Boric acid and sodium borate forms an acidic buffer (A mixture of weak acid and salt of weak acid and strong base). Place a pH electrode in the 0. Box 550 • 1560 Industry Road Hatfield, PA 19440 Phone: 215-412-8400 • Toll Free: 800-523-5874 Email: [email protected] Typically, 1-3% BSA is sufficient for most applications. 10. 0 g Na 2 B 4 O 7) or 9. 14 g/mol) 121. 10 H 2 O/L) and dilute to 1 L. 32. 4 M Sodium tetraborate decahydrate LB buffer, also known as lithium borate buffer, is a buffer solution used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. Borate buffer System Background There are various procedures for making a borate buffer. Example \(\PageIndex{1}\): pH of Solution Find the pH of the solution obtained when 1. For a buffer with pH 9. Make a solution of 0. Store up to 1 yr at 4°C. To make a purchase inquiry for this buffer, please provide your email Buffer Calculator. 2. com • infobangslabs. 05 m sodium tetraborate 10H 2 O Also make a solution of Boric acid (H3BO3). 5M, pH 7. Sep 26, 2023 · Procedure for Preparing 10x TBE (Tris Borate EDTA) Buffer. Buffer Calculator. Jun 18, 2023 · Learn how to prepare different types of buffer solutions like phosphate buffer solution, ammonia buffers, ammonium buffers, acetate buffers and citrate buffers from USP, BP and IP used in chemical analysis of Pharmaceutical ingredients. Tricine Buffer 0. 0. 5 with HCl (2 n ). Calculations for a Tris buffer solution will be used in the following example, but the methodology applies to any buffer solution. 5. Borate buffer solution Add 88 mL of 0. Test your borate level; Calculate your pool volume; Calculate how much borax to add or pool water to replace; Test Your Borate Level. e. Borate has a pK a value of 9. Usually the stock TBE electrophoresis buffer is prepared as a 5x or 10x concentrated solution which is diluted to 1x or 0. 0, 1×) Reagent. This means algae is a lot less likely to form in the water. 02, the molar concentrations of boric acid should be ____Mol/L. However, because TAE has the lowest buffering This buffer calculator provides an easy-to-use tool to calculate buffer molarity and prepare buffer solutions using the formula weight of the reagent and your desired volume (L, mL, or µL) and concentration (M, mM, or nM). Add 25. 60 M NaH2PO4, and 0. Adjust solution to desired pH (typically pH ≈ 7. 15 g of Sodium Phosphate Dibasic Dihydrate to the solution. Starting with the sodium borate solution, place a pH electrode in it while stirring and slowly add the boric acid solution 0. 2, Current Alkalinity from 70 to 220 ppm with a Desired pH from 6. The acid is weak hence the concentration of basic ion from acid is much less compared to the concentration of basic ion obtained from the salt which can ionize completely. Buffers regulate a constant pH range by neutralizing hydrogen ion effects which is very important for studies involving protein research, molecular biology, and characterization. 1 M, pH 6. 0, and the ability of activated and unactivated PMS to degrade organic pollutants has been explored [10], [32], [33], [34]. 288 M NaCH3COO. For most activities, 0. Add 0. 1 M of Boric acid and 1 M sodium hydroxide. 14 at 25 °C. To make a purchase inquiry for this buffer, please provide your email Sep 2, 2014 · The detection and assay of vitamin B-2 (riboflavin) was accomplished under aqueous conditions using sodium borate buffering at pH 7. 5 M Na2EDTA (pH 8. 2 M i. In swimming pools, borates are typically used in the form of sodium borate, boric acid, or borax. For higher levels (>500 Miller units), try 0. 6) 0. 0–10. 25 K, and a calculator that allows you to estimate pKa values of each buffer at temperatures form 3 o C to 37 o C, and concentrations from 1 to 500mM for "white background" buffers and 1 to 130mM for "brown background" buffers. Click here. 89889 Zeba Desalt Spin Columns, 2 ml, Mar 15, 2024 · As you can see from this chart, most of the borate is in the form of boric acid, which contributes to the total acidity. Add 887. 05M, (pH 8. It contains Tris, Boric acid, and EDTA and has pH ≈ 8. 14 g. 2, Borate buffer, traceable to NIST, ready to use, Thermo Scientific Chemicals $42. b. Use a stir bar and low heat to ensure that the boric acid dissolves fully. 1: The Action of Buffers. Sodium thiosulfate solution Calculate the concentrations of boric acid and sodium borate necessary to prepare a buffer solution of pH 9. , Tris). Yes. One method is to prepare a working buffer by using H 3 BO 3 and NaOH, or by using sodium tetraborate and HCl. Add boric acid and EDTA. 025 M Sodium tetra borate solution or 9. 4 g/L). 65 Specifications Borate buffer suitable for HPLC, LiChropur™, 0. 5 to 10. This buffer calculator provides an easy-to-use tool to calculate buffer molarity and prepare buffer solutions using the formula weight of the reagent and your desired volume (L, mL, or µL) and concentration (M, mM, or nM). 1 mL cells + 0. BO3 and NaH2BO3. To make a purchase inquiry for this buffer, please provide your email address below: Prepare 800 mL of dH2O in a suitable container. 01) resulted in a molar extinction coefficient of ε = 6486. 22-micron filter. To calculate the amount of buffer needed, please select a buffer from the Selection menu. suitable ionic concentration so that materials are neither extracted nor precipitated during fixation. Price: $41. mole/L. The buffer calculator can calculate various buffers which used to do experiment, including PBS Buffer, Acetic Acid-Sodium Acetate Buffer, Barbitone Sodium-HCl Buffer, Barbiturate Buffer, Borax-NaOH Buffer, Phosphate Buffer, Barbiturate Buffer, Glycine-HCl Buffer, Tris-HCl Buffer, KH 2 PO 4-NaOH Buffer, et al. This tool has helped you make litres of buffer (target 1,000,000 litres by September 2018) The main purpose of the site is to give access to the software written for the design of thermodynamically corrected buffers. 0126 M. 4) Carbonate-Bicarbonate Buffer (pH 9. Add 52. • Tech Tip #31: Calculate Dye:Protein (F/P) Molar Ratios Related Thermo Scientific Products 28384 BupH Borate Buffer Packs, 40 packs 28372 BupH Phosphate Buffered Saline Packs, 40 packs . Adjust to pH 8. 605 g of Citric Acid to the solution. Sodium Borate Buffer (1 M, pH 8. 421 g. 55 liter/mole. Makes corrections for the temperature at which the buffer will be used. 3. Mar 29, 2016 · How to calculate the pH of a 0. 5 or 9. The buffer at pH at 7. Buffers can react with both strong acids (top) and strong bases (bottom) to minimize large changes in pH. The TBE (Tris-Borate-EDTA) electrophoresis buffer contains Tris base, Boric acid, EDTA and has pH 8. Calculator for pH buffers. 421 g of Citric Acid to the solution. Add 18. After reconstitution, the composition of this buffer will be 50mM borate, pH 8. However, the slope of d vs. TBE buffer is typically used for agarose DNA electrophoresis. A 10x Tris-Borate (TB) electrophoresis buffer can be prepared by dissolving 108 g Tris base, and 55 g Boric acid in water to a final volume of 1000 ml. This wide range is due to phosphoric acid having 3 dissociation constants, (known in chemistry as a triprotic acid) allowing for formulation of buffers near each of the pH levels of 2. 274 g of Sodium carbonate (anhydrous) to the solution. 355 M CH3COOH and 0. Borate Buffer solution Add 88 mL 0. Phosphate buffer is highly water soluble and has a Standard Buffer Solutions —Standard solutions of definite pH are readily available in buffer solutions prepared from the appropriate reagents. 245 g of Potassium Phosphate Monobasic to the solution. In a flask/beaker keep 900 ml of distilled water, add 108 g of Tris and 55 g of Boric acid, and stir it well with a magnetic stirrer. 5 M, pH 9. Tris base (mw: 121. Apache/2. [sodium borate] = 0. 21, or 12. Sodium hydroxide solution 6 N Dissolve 240 grams of sodium hydroxide (NaOH) in 1 liter of ammonia free water. suitable osmolarity so that cells and organelles neither swell nor shrink during fixation. This calculator is valid for a buffer of a weak acid and it's conjugate base of the same system. Sterilize by vacuum-filtration with a 0. 8 mg of Potassium Phosphate Monobasic to the solution. 282 g of Potassium phosphate dibasic to the solution. This form calculates the amount of two buffer components necessary to achieve a buffer at a given pH and strength. A simple phosphate buffer is used ubiquitously in biological experiments, as it can be adapted to a variety of pH levels, including isotonic. Oct 10, 2017 · To calculate the activity of Cl −, a C l −, the mean molar activity coefficient (γ±) for different Cl − concentrations in deaerated borate buffer solution were used [33]. Add 7. 025M Sodium tetraborate (Na 2 B 4 O 7) solution (5. The Tris-glycine buffer system (pH 9. 1 M. At 77ºF/25ºC: Bicarbonate has a pK a value of 6. Supplier: Electron Microscopy Sciences. 5) Citrate Buffer (0. Add 352. Dec 4, 2018 · Boric acid is the predominant form at the typical pH range in swimming pools and spas (pH 7. Note that here [CH3COOH] = [CA] and [CH3COONa] = [CB]. Solution B (0. Add 40 ml of 0. Materials. Calculate buffer molarity and get buffer solution recipes for Tris, sodium phosphate, and many others. 5 g of Potassium phosphate dibasic to the solution. The TBE buffer (Tris Borate EDTA buffer) is used in DNA/RNA electrophoresis. 0 or higher. 14 g of Tris base to the solution. The pH – Alkalinity Adjustment Tool is different from the Pool Acid Dose Calculator because the Pool Acid Dose Calculator only works for lowering pH and alkalinity and only from Current pH 7. Our buffers are ideal for maintaining a constant pH. 5 M, pH 10. c. Publish a Booklet on Buffers? We are frequently asked questions on the use of buffers that we offer to research laboratories. 140 M; find Supelco-89273 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Jun 30, 1986 · Make the transfer using as small a quantity of water as possible and do not exceed a total volume of 50 mL. Adjust solution to final desired pH using HCl or NaOH. 0 to 6. E seemed similar. 1 8. Feb 1, 2021 · In many studies, borate buffer has been frequently used as the buffer solution to maintain the pH at 7. The absorbance spectrum of riboflavin was determined at different pH values utilizing several buffers. Borate Buffer, pH 9. 274 g. A generic biologic buffer such as PBS or PBS-Tween ® may be utilized for Jun 19, 2020 · Equation \(\ref{8}\) is called the Henderson-Hasselbalch equation and is often used by chemists and biologists to calculate the pH of a buffer. Add distilled water until the volume is 1 L. 140 M; find Supelco-89273 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Assay Buffer Prepare by dissolving contents of the BupH Borate Buffer Pack in 500mL distilled water. 5M EDTA (pH 8. A 1x TBE electrophoresis buffer contains 89 mM Tris base, 89 mM Boric acid and 2 mM EDTA. Add to cart. Method: Filtered through 0. 2 M solution of sodium acetate; the conjugate pair here is acetic acid HAc and its conjugate base, the acetate ion Ac –. pH Buffer Strength mM. 0 - 11. 2 6. Buffer solution pH 9. This equation is also known as Henderson-Hasselbalch equation. 0875 M. pH = pKa + log ( [ NaH2BO3] / [H3BO3] Question: Calculate and formulate an ophthalmic buffer solution of boric acid (pKa=9. Oct 1, 2015 · The result was compared with literatures, in which the passive film thicknesses on 316L SS in borate buffer solution were measured by capacitance calculation [5] and Auger electron spectroscopy (AES) [29]. Add 16. 250 mL. 5 g of Sodium tetraborate decahydrate in 900 ml. To prepare 1L of 10x solution, you need: 108 g Tris base; 55 g boric acid; 40 mL 0. O. 100 M NaOH is added to 200 mL of the buffer. zr iu ck qt ss ao dv dg pu jw